Biological control of potato soft rot caused by Erwinia carotovora subsp. carotovora

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NATURAL EXTRACTS OF MUSHROOM AND GARLIC AS BACTERICIDE ALTERNATIVES AGAINST POTATO SOFT-ROT BACTERIA, Erwinia carotovora subsp. carotovora

Current study aimed to search for safe and effective natural products to control the potato soft-rot disease, Erwinia carotovora subsp. carotovora. Antibacterial efficiency of edible mushroom extracts (MEs) and garlic essential oil (EO) were compared with two bactericides; streptrol and oxolinic acid as well as two fungicides; mancopper and copper oxychloride. Bioassay experiments were conducte...

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Biological Control of Erwinia carotovora ssp. carotovora by Streptomyces Species

Ten isolates of Erwinia carotovora ssp. carotovora (Ecc) were isolated from infected potato tubers of Picasso, Sante, and Nevskiy varieties collected from different regions in Kyrgyzstan. Isolates were identified as Erwinia carotovora ssp. carotovora (Ecc) by standard bacteriological techniques and pathogenicity tests on tubers and also by PCR analyses. Tests on the pathogenicity of E. carotovo...

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Efficient transformation of Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica.

We used a modified version of the method of Hanahan (D. Hanahan, J. Mol. Biol. 166:557-580, 1983) to transform Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica with the plasmids pBR322, pBR325, and pAT153. The transformation frequency ranged from 1 X 10(2) to 4 X 10(4) colonies per micrograms of plasmid DNA. The nature of these transformants was confirmed by plasmid ana...

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Identification and cloning of an Erwinia carotovora subsp. carotovora bacteriocin regulator gene by insertional mutagenesis.

Avirulent Erwinia carotovora subsp. carotovora CGE234-M403 produces two types of bacteriocin. For the purpose of cloning the bacteriocin genes of strain CGE234M403, a spontaneous rifampin-resistant mutant of this strain, M-rif-11-2, was isolated. By Tn5 insertional mutagenesis using M-rif-11-2, a mutant, TM01A01, which produces the high-molecular-weight bacteriocin but not the low-molecular-wei...

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Regulation of the expression of prtW::gusA fusions in Erwinia carotovora subsp. carotovora.

Erwinia carotovora subsp. carotovora, a Gram-negative phytopathogenic bacterium, secretes an extracellular metalloprotease, PrtW. Previous results demonstrated that protease activity is necessary for the normal progression of disease symptoms caused by this bacterium. The present study revealed that the prtW gene constitutes an independent transcriptional unit. It is demonstrated that introduct...

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ژورنال

عنوان ژورنال: Egyptian Journal of Biological Pest Control

سال: 2018

ISSN: 2536-9342

DOI: 10.1186/s41938-018-0100-x